PATENTS APPLIED AND GRANTED

DESIGN PATENTS GRANTED:

1.      Pronil-CVPTM:

Design-Patent Number-47130, Certificate of Registration of Design No. 273619 dated 27.03.15, Certificate No.47130 dated 06.07.16.  This design patent is useful to prevent recurring pre-partum Prolapse Cervix and Vagina in Bovines

Inventors:N Srivastava, Megha Pande, H Kumar

2.      Prolacure-UtPTM:

Design-Patent Number- 47140, Certificate of Registration of Design No. 270693 dated 27.03.15, Certificate No. 47140 dated 26.08.16.

Inventors:Megha Pande, N Srivastava, S Mehrotra, AK Mathur

TECHNOLOGIES FILED FOR PATENT:

 DEVELOPMENT OF A RAPID USER FRIENDLY SINGLE TUBE PCR BASED TOOL FOR DETECTION OF BOVINE LEUKOCYTE ADHESION DEFICIENCY (BLAD) CARRIER IN CATTLE:

A single tube tetra ARMS PCR based diagnostic strategy developed for detection of BLAD carrier in cattle. The test is rapid, economical and user friendly. The test has been utilized to screen Frieswal bulls for identifying BLAD carrier before semen collection.  A Patent has been filed with application number 1073/DEL/2015.

Description of the technology: Bovine Leukocyte Adhesion Deficiency (BLAD) is a lethal autosomal recessive disorder characterized by greatly reduced expression of the heterodimeric integrin adhesion molecules on leukocytes resulting in multiple defects in leukocyte function and the affected calves usually dies within 2-4 months of age. The developed tetra primer single tube PCR based genotyping assay can be used for the detection of BLAD positive animals and can be easily adapted in laboratories for rapid detection of carrier animals. The test can be completed in 2 to 2 and half hours and does not need expensive.

Inventors:R.R.Alyethodi, Umesh Singh, Sushil Kumar, Rajib Deb, Rani Alex, Sheetal Sharma, B.Prakash.

DEVELOPMENT OF TETRA PRIMER DISPLACEMENT PCR ASSAY FOR DETECTION OF COMPLEX VERTEBRAL MALFORMATION (CVM) CARRIERS IN CATTLE:

Description of the technology: Complex vertebral malformation (CVM), a genetic lethal syndrome in Holstein calves. The developed tetra primer displacement PCR based genotyping assay can be used for the detection of CVM positive animals and can be easily adapted in laboratories for rapid detection of carrier animals. The test has the following usefulness/advantages over the existing methodologies viz. 1. Rapidity – Depending on the DNA polymerase used, the test can be completed in one and half hours (displacement PCR based) OR two and half hours (Normal PCR). 2. User friendliness – It is easy to perform, 3. Economical – It does not need expensive reagents such as real time PCR machine, restriction enzyme, reagents for PAGE, Staining solution etc., 4. Specificity - Assay employs tetra primer pairs with deliberately incorporated mutation. A patent has been filed with application number 201611034916.

Inventors:R.R. Alyethodi, Umesh Singh, Sushil Kumar, Rani Alex, Rajib Deb, Raja T V, Gyanendra S Sengar, B. Prakash.    

A COLOR BASED ASSAY FOR DIFFERENTIATING COW VS GOAT MILK/MEAT:

Description of the technology:A post PCR color based differentiation of cow vs goat milk and meat samples has been developed. The test does not require downstream PCR processing and handling of carcinogenic dyes. Moreover, it is rapid and can be completed within 2-3 hour including genomic extraction from milk/meat samples. The test can detect 2% adulterated milk or 1 mg meat from either cow or goat sources. A Patent has been filed with application number 1901/DEL/2015.

Inventors:Rajib Deb, Rani Alex, Sushil Kumar, Umesh Singh, T.V.Raja, R.R.Alyethodi, GyanendraSengar, B.Prakash.

DEVELOPMENT OF AN IN-HOUSE BUILT LAMP ASSAY FOR RAPID DETECTION OF COW COMPONENTS ADULTERATED IN BUFFALO MILK/MEAT.

An in-house built rapid, economical and user-friendly LAMP assay protocol to identify the buffalo milk/meat samples adulterated with cow milk/meat was developed. This test can be completed with in 1 hour 45 mintes and can detect upto 5% adultrated cow DNA in buffalo counter parts. A Patent has been filed with application number 3870/DEL/2015.

Description of the technology: A loop-mediated isothermal amplification (LAMP) assay for rapid and specific detection of cow specific DNA segment under isothermal conditions was developed. The technique requires a set of conditions and primers different from those used for normal PCRs. The LAMP reaction typically occurs over 30 to 60 min. with temperatures ranging from 60 to 65°C under isothermal conditions and can be conducted with a simple heating block instead of a thermocycler thus avoiding the standard lengthy PCR protocols. LAMP assays have now been reported for detection of various pathogens; however, its application for species identification is not yet reported so far. In the present study, we developed an in-house built rapid, economical and user-friendly LAMP assay protocol to identify the buffalo or goat milk/meat samples adulterated with cow milk/meat. The developed LAMP assay can be used for the rapid detection of cow milk/meat DNA in buffalo or goat samples and can be easily adapted in laboratories.

Inventors:Rajib Deb, Umesh Singh, Sushil Kumar, A. K. Das, T. V.Raja, R.R.Alyethodi, Rani Alex, Gyanendra Sengar, B. Prakash.

IDENTIFICATION OF FUNCTIONAL INTERNALRIBOSOMAL ENTRY SITES (IRES) AT BOVINEHEAT SHOCK PROTEIN 90.

A putative IRES wasidentified in bovine heat shock protein genefor the first time, which was showed to befunctional. The identified bovine heat shockprotein IRES was used to develop an artificialexpression cassettes for simultaneoustranslation of two gene from a same readingframe. An Indian patent has been filed withapplication number 201711042562 dated28/11/2017.Patent has been filed and published (The PatentOffice Journal no. 02/2019, dated 11/01/2019, Page No: 1129).

Inventors:Rajib Deb, Umesh Singh, Gyanendra Singh Sengar, Basavraj Sajjanar (NIASM,Baramati), Sushil Kumar, Saugata Hazra (IITRoorkee,UK), Rani Alex, T V Raja, Rafeeque Rahman Alyethodi, Ashish Kumar, A K Das, BPrakash.

DETECTION OF ADULTERATION OF CAMEL MILK WITH COW MILK OR VICE-VERSA.

The technology is useful for detecting cow milk adulteration in camel milk and vice versa at a minimum level of 1%. The milk is considered as a functional food used as a natural remedy in many part of world for the management of a number of human diseases for which it`s purity is important. The Prevention of Food Adulteration Act (PFA) stipulates the supply of pure milk to the consumers. Presently, due to high market demand and less supply of camel milk, it is being adulterated with cow milk. As it is well known, food adulteration is a global concern and developing countries like India are at higher risk. The kit is less expensive, user friendly, rapid and specific in detection of adulteration of camel milk with cow milk or vice-versa. The test described can be completed less than 4 hours. The stability of the reagents in both the kits can be more than six months in normal refrigerated temperature.

Inventors:Rajib Deb, Amod Kumar, T V Raja, Umesh Singh, Sushil Kumar, N V Patil, Gyanendra S Sengar