The semen quality
control laboratory was established in 2005 to ensure the availability of
quality semen to the stakeholders. The frozen semen from the bulls is
thoroughly evaluated based on the guidelines of CMU, GOI for production of
quality germplasm. Each batch of frozen semen is evaluated through a series of
quality tests and semen samples that pass the minimum prescribed standards as
per cut-off values detailed below are only stored for future use. The major
tests include hypo osmotic swelling test (HOST), Incubation Test, Acrosome
integrity, viability and morphology (live-dead percentage of sperms and sperm
abnormalities), sperm concentration and microbial load. Besides, the frozen
semen samples are also get tested for the IBR once in every week through
molecular genetics laboratory of the institute. The laboratory is also working
on assessing the antimicrobial sensitivity/resistance pattern of common
microbes present in semen.
Table: Quality parameters
and their cut-off values
S. No |
Quality parameters |
Cut-off value |
1. |
Bacterial
Load |
5000
CFUs /ml |
2. |
Hypo
Osmotic Swelling Test (HOST) |
≥
40% |
3. |
Incubation
Test |
Standard
drop in motility by 10% after every 30 minutes |
4. |
Acrosome
integrity (Fresh semen) |
≥
70% |
5. |
Percent
Intact Acrosome (PIA) |
≥
65% |
6. |
Sperm
Concentration |
20
million spermatozoa per dose (0.25
ml Mini straw) |